Performance Evaluation of AODV Routing Protocol in VANET with NS2

In intelligent transportation systems, the collaboration between vehicles and the road side units is essential to bring these systems to realization. The emerging Vehicular Ad Hoc Network (VANET) is becoming more and more important as it provides intelligent transportation application, comfort, safety, entertainment for people in vehicles. In order to provide stable routes and to get good performance in VANET, there is a need of proper routing protocols must be designed. In this paper, we are working with the very well-known ad-hoc on-demand distance vector (AODV) routing protocol. The existing Routing protocol AODV-L which is based on the Link expiration time is extended to propose a more reliable AODV-AD which is based on multichannel MAC protocol. For the performance evaluation of routing protocols, a simulation tool ‘NS2’ has been used. Simulation results show that the proposed AODV-AD protocol can achieves better performances in forms of high Route stability, Packet Delivery ratio and packet loss rate than traditional AODV-L and traditional AODV

Comparison of stressful life events among family caregivers of cancer, mental retardation and schizophrenia patients leading to stress, anxiety and depression and its effect on quality of life

Background: Mental retardation (MR), schizophrenia and cancer are chronic disorders, requiring long term treatment along with family support. Principal caregiver is usually nonprofessional family person who play a pivotal role in supporting the patient medically, emotionally and financially which in turn affects their own health and quality of life. To identify stressful life events, level of anxiety, stress, depression and Quality of life in these family caregivers (FC), this study was planned.Methods: After gaining consent, the FC were assessed on Presumptive Stressful Life Events Scale (PSLE), Depression Anxiety Stress Scale (DASS) and WHO Quality of Life -Bref Scale (WHOQOL-Bref). Data so gained was analyzed by SPSS-21 and results were drawn.Results: 66 FC of cancer patients, 39 FC of MR patients and 53 FC of Schizophrenia patients were assessed. All three groups were found to be matched socio-demographically. On PSLE Mean of total stressful life events for cancer FC was 7±1.5, for MR 6.02±1.5 and for schizophrenia FC was 5.75±1.70 and this difference was statistically significant. Mean of total undesirable life events was 5.31±1.3, 4.33±1.34 and 3.86±1.46 respectively and this difference was also statistically significant. Similarly difference in mean stress score of total life events and for undesirable events was also statistically significant. On DASS scale no statistically significant difference was found within the groups. Quality of life was significantly low depicting high impact on physical health and social life of these care givers.Conclusions: Family Caregivers of cancer, mental retardation and schizophrenia groups are highly affected and there is urgent need to address these issues for early diagnosis and treatment

Membrane-associated proteomics of chickpea identifies Sad1/UNC-84 protein (CaSUN1), a novel component of dehydration signaling

Dehydration affects almost all the physiological processes including those that result in the accumulation of misfolded proteins in the endoplasmic reticulum (ER), which in turn elicits a highly conserved signaling, the unfolded protein response (UPR). We investigated the dehydration-responsive membrane-associated proteome of a legume, chickpea, by 2-DE coupled with mass spectrometry. A total of 184 protein spots were significantly altered over a dehydration treatment of 120 h. Among the differentially expressed proteins, a non-canonical SUN domain protein, designated CaSUN1 (Cicer arietinum Sad1/UNC-84), was identified. CaSUN1 localized to the nuclear membrane and ER, besides small vacuolar vesicles. The transcripts were downregulated by both abiotic and biotic stresses, but not by abscisic acid treatment. Overexpression of CaSUN1 conferred stress tolerance in transgenic Arabidopsis. Furthermore, functional complementation of the yeast mutant, slp1, could rescue its growth defects. We propose that the function of CaSUN1 in stress response might be regulated via UPR signaling

Abnormal Complement Activation and Inflammation in the Pathogenesis of Retinopathy of Prematurity

Retinopathy of prematurity (ROP) is a neurovascular complication in preterm babies, leading to severe visual impairment, but the underlying mechanisms are yet unclear. The present study aimed at unraveling the molecular mechanisms underlying the pathogenesis of ROP. A comprehensive screening of candidate genes in preterms with ROP (n = 189) and no-ROP (n = 167) was undertaken to identify variants conferring disease susceptibility. Allele and genotype frequencies, linkage disequilibrium and haplotypes were analyzed to identify the ROP-associated variants. Variants in CFH (p = 2.94 x 10(-7)), CFB (p = 1.71 x 10(-5)), FBLN5 (p = 9.2 x 10(-4)), CETP (p = 2.99 x 10(-5)), and CXCR4 (p = 1.32 x 10(-8)) genes exhibited significant associations with ROP. Further, a quantitative assessment of 27 candidate proteins and cytokines in the vitreous and tear samples of babies with severe ROP (n = 30) and congenital cataract (n = 30) was undertaken by multiplex bead arrays and further validated by western blotting and zymography. Significant elevation and activation of MMP9 (p = 0.038), CFH (p = 2.24 x 10(-5)), C3 (p = 0.05), C4 (p = 0.001), IL-1ra (p = 0.0019), vascular endothelial growth factor (VEGF) (p = 0.0027), and G-CSF (p = 0.0099) proteins were observed in the vitreous of ROP babies suggesting an increased inflammation under hypoxic condition. Along with inflammatory markers, activated macrophage/microglia were also detected in the vitreous of ROP babies that secreted complement component C3, VEGF, IL-1ra, and MMP-9 under hypoxic stress in a cell culture model. Increased expression of the inflammatory markers like the IL-1ra (p = 0.014), MMP2 (p = 0.0085), and MMP-9 (p = 0.03) in the tears of babies at different stages of ROP further demonstrated their potential role in disease progression. Based on these findings, we conclude that increased complement activation in the retina/vitreous in turn activated microglia leading to increased inflammation. A quantitative assessment of inflammatory markers in tears could help in early prediction of ROP progression and facilitate effective management of the disease, thereby preventing visual impairment

Performance Evaluation of AODV Routing Protocol in VANET with NS2

In intelligent transportation systems, the collaboration between vehicles and the road side units is essential to bring these systems to realization. The emerging Vehicular Ad Hoc Network (VANET) is becoming more and more important as it provides intelligent transportation application, comfort, safety, entertainment for people in vehicles. In order to provide stable routes and to get good performance in VANET, there is a need of proper routing protocols must be designed. In this paper, we are working with the very well-known ad-hoc on-demand distance vector (AODV) routing protocol. The existing Routing protocol AODV-L which is based on the Link expiration time is extended to propose a more reliable AODV-AD which is based on multichannel MAC protocol. For the performance evaluation of routing protocols, a simulation tool ‘NS2’ has been used. Simulation results show that the proposed AODV-AD protocol can achieves better performances in forms of high Route stability, Packet Delivery ratio and packet loss rate than traditional AODV-L and traditional AODV

Patient’s Perspective on Barriers and Facilitators in Management of Type 2 Diabetes: A Systematic Review

Objective: The purpose of this review is to summarize existing knowledge regarding various barriers and facilitators in the management of Type 2 diabetes from the perspective of patients. Method: A comprehensive electronic search was conducted which included three sources PubMed, Embase, and Web of Science, and studies from 1997 to 2021 were included. Around 1687 papers were screened and 46 studies were included in the final analysis. Result: Three major areas of barriers and facilitators in Type 2 diabetes management were identified: Individual factors: factors which are emerging due to a patient’s personal ability to take care of type 2 diabetes (adherence to diet, physical activity, self-monitoring of blood glucose, medication adherence, psychological factors: this includes emotional factors, beliefs and attitude of patients. Organizational factors: it includes diabetes education factors, economic constraints and health care provider’s issues and social factors: this includes factors associated with family, friends, and others social issues. Conclusion: Identifying barriers to diabetes management is vital to improve the quality of care of diabetes patients, including the improvement of glycemic control, and diabetes self-management. Further research which considers these barriers and facilitators are necessary for developing interventions for individuals with type 2 diabetes

Antimicrobial potential of polyvinyl pyrollidone stabilized silver nanoparticles synthesized by Sphingobacterium multivorum

364-372Biological synthesis of nanoparticles is emerging as a promising solution to tackle issues associated with conventional synthesis methods. Silver nanoparticles (AgNPs), owing to their unique physiochemical and antimicrobial properties, attract more attention. In this study, we have made an attempt to develop ecofriendly and stable AgNPs with antimicrobial potential. AgNPs were synthesized using Gram negative Sphingobacterium multivorum supernatant and characterized by UV-Visible spectrophotometric analysis, X-Ray Diffraction analysis, Transmission electron microscopy, Fourier transform infrared and Dynamic Light scattering. Biosynthesized AgNPs exhibited broad spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria. Minimum inhibitory concentration was detected 19.5 μg for E. coli and Staphylococcus aureus. AgNPs have exhibited significant synergistic effect with all the selected antibiotics. The results demonstrated a rapid, economic and ecofriendly method for the synthesis of stable AgNPs and further evaluation of the antimicrobial activity against Gram-positive and Gram-negative bacteria

Optimization of Protein Isolation and Label-Free Quantitative Proteomic Analysis in Four Different Tissues of Korean Ginseng

Korean ginseng is one of the most valuable medicinal plants worldwide. However, our understanding of ginseng proteomics is largely limited due to difficulties in the extraction and resolution of ginseng proteins because of the presence of natural contaminants such as polysaccharides, phenols, and glycosides. Here, we compared four different protein extraction methods, namely, TCA/acetone, TCA/acetone–MeOH/chloroform, phenol–TCA/acetone, and phenol–MeOH/chloroform methods. The TCA/acetone–MeOH/chloroform method displayed the highest extraction efficiency, and thus it was used for the comparative proteome profiling of leaf, root, shoot, and fruit by a label-free quantitative proteomics approach. This approach led to the identification of 2604 significantly modulated proteins among four tissues. We could pinpoint differential pathways and proteins associated with ginsenoside biosynthesis, including the methylerythritol 4–phosphate (MEP) pathway, the mevalonate (MVA) pathway, UDP-glycosyltransferases (UGTs), and oxidoreductases (CYP450s). The current study reports an efficient and reproducible method for the isolation of proteins from a wide range of ginseng tissues and provides a detailed organ-based proteome map and a more comprehensive view of enzymatic alterations in ginsenoside biosynthesis